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PubMed , Antioxidants & Redox Signaling , vol

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10.1097/wnr.0b013e32830b3677 146 MarquesS

Proteins were transferred to polyvinylidene difluoride (PVDF) membranes

Additionally, after denaturation by boiling in loading buffer, the total protein in cell lysate was separated by 15% SDS-PAGE, transferred from gel to nitrocellulose membrane (Millipore, USA) at 250 mA for 2 h, and blocked with by 5% non-fat milk for 1 h