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however, this can substantially distort metal coordination geometry[ 107 , 152 ] and prevents valuable information regarding SOD1s native posttranslational modifications from being collected

The ABI 7500 real-time PCR detection system was used to perform quantitative RT-PCR (qRT-PCR) following the manufacturers instructions and an SYBR green Premix Ex TaqTM kit (Takara)

Additionally palmitate metabolism may give rise to ceramide synthesis, ceramide being a key component of the signal transduction pathway for ROS-induced apoptosis [73]