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2.6 Culture and polarization of macrophages Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls by Ficoll-Paque PREMIUM density gradient (GE Healthcare, USA) centrifugation

This increase was maintained following treatment with LPS

We found that Ac-DEVD-CHO, a caspase 3 inhibitor, better rescued cytotoxicity of NSBSO against B16 cells when comparing with the caspase 1 inhibitor (Z-YVAD-FMK) (Additional file 1: Fig