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This dye exhibits two distinct emission peaks: red fluorescence from J-aggregates, indicative of hyperpolarized mitochondria (high membrane potential), and green fluorescence from JC-1 monomers, associated with depolarized mitochondria (low membrane potential)

Thus, the alteration of GABA-glutamate equilibrium resulted in a sustained high GABA/glutamate ratio in JEV-infected brain, which is secondary to the aberrant glutamine-glutamate metabolism described before

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Their discovery grew out of gain-of-function screens performed by Elledge and colleagues, which identified suppressors of the yeast cell cycle mutant Cdc4