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Consistently, chemogenetic activation of Crus I Purkinje cells projecting to the VTA has been shown to attenuate depressive-like behaviors in chronically stressed mice, reinforcing the notion that cerebellar circuits contribute to the regulation of affective states and mood-related comorbidities commonly observed in chronic pain (75)

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Cells were lysed and GSTP1 was immunoprecipiated with agarose beads conjugated to mouse anti-V5 antibody (V5-10), subjected to click chemistry, resolved on 11% SDS-PAGE and transferred to a PVDF membrane

The dominant documented experience in these weeks was appetite change and titration-phase GI effects rather than large body-composition shifts