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Ferreira SC, Bruns RE, Ferreira HS, Matos GD, David JM, Brandao GC, da Silva EP, Portugal LA, Dos Reis PS, Souza AS, Dos Santos WN (2007) Box-Behnken design: an alternative for the optimization of analytical methods

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J Endocrinol Invest 44(9):18271835

Preparation Condition 3 (EDTA 80 o C deproteinization): Deproteinization status does not affect measurement of GSH in EDTA-treated plasma We found no differences in total GSH (Fig