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Pain Relievers (e.g., Ibuprofen, Aspirin) Usually No Deferral : Over-the-counter pain relievers like ibuprofen or acetaminophen are usually fine, but its important to disclose when you last consumed them during your screening session

Less common: Temporary skin discoloration at the injection site

doi:10.1007/s11892-019-1150-5 78

Performance characteristics Synonyms ALT Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01-0.83 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 75 min Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 2.3% Other instruments required Pipettor, Vortex mixer, Centrifuge Storage -20 C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L)

When measurement of loss of mitochondrial membrane potential is performed together with cell viability, the former is detected only in dead cells in PQ treated cells (on the contrary for MPTP and Rotenone is detected in alive cells suggesting this event precedes cell death)