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Hormones 19:1524 Stanishevska NV (2018) Modern concept of biological identification of selenoproteins

An equal volume of 250 mmol/L sucrose, 30 mmol/L HEPES, pH 7.4 containing complete mini protease inhibitor cocktail (Roche) was added, followed by centrifugation for 5 min at 1,000 g and 4C to pellet nuclei and remaining intact cells