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Alcohol intake in relation to non-fatal and fatal coronary heart disease and stroke: EPIC-CVD case-cohort study

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After blocking with 5% BSA (Sigma-Aldrich) in phosphate-buffered saline, 50 L of mouse serum diluted 1:100, 1:300, 1:900, 1:1,800, 1:3,600, 1:7,200, 1:144,000, or 1: 288,000 was added to each well

If any of the variables were not normally distributed (as verified by a Shapiro-Wilk test), then a Mann-Whitney t -test was used to compared groups

Bhandari et al., 2017b]