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Co-immunoprecipitation The recombinant GST-tagged Nanobody against GFP 69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to the manufacturers instructions

Depletion of Trim26 in hepatocytes alters liver metabolic equilibrium, leading to glucose metabolic dysfunction, lipid buildup, hepatic inflammation, and fibrosis, all of which accelerate the progression of the NASH phenotype [42]

Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity

Thus, it is recommended to obtain bodyweight alongside collection of the bio-measures

As multi-omics technologies advance, extensive genomic data, even at the single-cell level, is increasingly accessible (Atherine et al., 2022)