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Briefly, the coding sequence (CDS) of HrGSTm1 was cloned into the pET-32a(+) vector (TaKaRa, Shiga, Japan) with Bam HI and Hin dIII restriction sites, then transformed into competent Escherichia coli (DE3) cells (AxyGen, Shanghai, China) and cultured at 37 C for 24 h

The gut microbial metabolite trimethylamine N-oxide aggravates GVHD by inducing M1 macrophage polarization in mice

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