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Oxidative stress and antioxidant biomarkers in clinical and experimental models of non-alcoholic fatty liver disease

The eluted sample was incubated at 65 C overnight to reverse the crosslinking, and DNA was extracted using a TIANquick Midi purification kit (TIANGEN, DP204, Beijing, China) and subjected to PCR analysis

More importantly, oxidant enzymes including mitochondrial electron transport chain complex I, complex II, complex III, as well as SODs, xanthine oxidase (XO), NO synthases (NOS), cytochrome p 450 enzymes (CYP), NADPH oxidases (NOX) and lipoxygenase (LOX), generate O 2 radicals which are quickly converted into H 2 O 2 , OH and other radicals [26]

ROO primarily targets the polyunsaturated fatty acids (PUFA) found in DNA bases and cell membranes, but it also targets almost all important groups of biomolecules