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The His tag is one of the most widely used purification tags, and it offers several advantages (Kimple and Sondek, 2004

Selective vulnerability of neurons to acute toxicity after proteasome inhibitor treatment: implications for oxidative stress and insolubility of newly synthesized proteins

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For example, gemcitabine thermosensitive hydrogels or microspheres are used for intraperitoneal perfusion to treat peritoneal metastasis, which can form a drug sustained release reservoir in the peritoneal cavity, prolong local exposure time and reduce systemic toxicity (Xu et al., 2016)