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Ying L, Li N, He Z, Zeng X, Nan Y, Chen J, et al

HepG2-hNTCP cells were cultured in primary hepatocyte maintenance medium (PMM) for 6 h, then inoculated with 1000-fold genomic equivalents of HBV and incubated in PMM containing 4% PEG 8000 (Sigma -Aldrich, 89610) at 37 C for approximately 16 hours