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Titration experiments revealed that the optimal GSNO concentration corresponded to approximately 50% cysteine labeling, which is consistent with the reaction requiring both S-nitrosylated cysteine and free thiolate

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This mechanism makes Milk Thistle Phytosome an excellent complementary therapy to NAC (N-Acetyl-l-Cysteine), as both work synergistically to replenish the body's master antioxidant reserves and support robust Phase II liver detoxification