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Cells were cultured using AIM-V (Thermo Fisher Scientific) supplemented with 10% FBS, 100 U ml 1 penicillin, 100 g ml 1 streptomycin and 2 mM l-glutamine under humidified conditions in 5% CO 2 at 37 C

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Modulation of myocardial mitochondrial mechanisms during severe polymicrobial sepsis in the rat