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Abstract The purpose of this study was to investigate the effect of different times return to activity on tendon healing after Double Kessler method suture in rats with Achilles tendon rupture

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Lithium induces autophagy by inhibiting inositol monophosphatase

Cells were resuspended in lysis buffer (50 mM NaCl, 50 mM Tris 7,4, 1 mM EDTA, 0.1% NP40, 1 mM DTT, and protease inhibitor cocktail), and following sonication, the lysate was clarified by centrifugation

These effects typically relate to reduced food intake and caloric adaptation