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Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb

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Reconstitute closer to what the cycle needs

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In the unlikely event of significant copper toxicity, treatment involves IV chelation therapy using EDTA, which binds to excess copper and allows it to be excreted through the kidneys