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Post-centrifugation at 350 g for 5 minutes, cells were lysed in 3 ml ACK buffer for 5 minutes, then diluted to 35 ml with RPMI with HEPES buffer and without antibiotics (RPMI-MGIA) and centrifuged again

Potential side effects (usually related to the benzyl alcohol preservative) include: Allergic reactions (rare) Local irritation at injection site In neonates or very small infants, benzyl alcohol can cause toxicity (gasping syndrome) and should NOT be used

High-dose NAC inhalation therapy shows promise in rectifying the oxidant-antioxidant imbalance observed in lung tissue of IPF patients, thereby ameliorating fibrotic progression [180]

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