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24 hours after transfection, cells were treated with 1 g/mL puromycin (Invitrogen) for 72 hours to select for cells that contained the plasmid

Treatment Vitamin B12 supplementation: Since it is an essential nutrient, it is best to add multivitamin supplementation to avoid all deficiencies

Heliyon 7 , e06124 (2021)

doi:10.1016/j.cell.2021.04.041

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