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Lu Q, et al

[96] demonstrated heterotypic chronic stress (HeCS) could induce visceral hypersensitivity and sensitize colon-specific dorsal root ganglion (DRG) neurons through improving expression of nerve growth factor (NGF) in colonic muscularis externa and mucosa/submucosa

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Each genotype consisted of four treatment groups as follows: the first group served as a positive control with non-injected eggs (Control), the second group was injected with 0.5 mL 0.75% saline solution (NaCl) only and served as the sham control (Saline), the third group was injected with 5 mg of DL-Met (0.5 mL of the prepared solution) (DL-Met), and the fourth group was injected with 5 mg of L-Met (0.5 mL of the prepared solution) (L-Met)