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Liver histological staining and immunolabelling Pieces of liver tissue from postmortem PBS-perfused mice (at least 3 animals per group) were fixed in 4% paraformaldehyde for 12 h, stored in 70% ethanol, and embedded in paraffin blocks and sectioned using an RM2165 microtome (Leica Microsystems)

10.1002/jcb.29237 J Cell Biochem

GHK has been shown to enhance extracellular matrix (ECM) activity in both dermal and pulmonary fibroblasts

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Despite the above claims, there are no clinical trials showing that coffee enemas can improve health