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Cells were washed three times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for one hour in the dark

The past 10 years, however, have seen the development of enzyme therapy for arginase deficiency and gene therapy for ornithine transcarbamylase deficiency

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