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Remove when almost melted and continue to stir until completely melted

In summary, our in-silico trajectory analysis suggests a developmental lineage of human secretory cells, driven by sequentially activated TFs, which transitions through functional intermediates (club cells) to culminate in a multi-functional mucus secretory cell 29,30,31,32 , although all cells in this lineage need not reach this mucus secretory endpoint, depending on internal or external differentiation cues

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