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Kayser-Fleischer rings (indicated by the black arrow) are gold or greenish gold rings around the cornea

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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

Many findings are encouraging, but no skincare ingredient should be viewed as a guaranteed solution