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methodology, A.B., R.M., M.T., F.M.H., A.H., E.S.E., E.K., A.F., F.I.A.E.-E., S.M.M

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Cytosolic and nuclear NAD + are often considered a shared pool as NAD + diffuses through nuclear pores 58 , and importantly, the concentrations of NAD + in the cytosol, nucleus, and mitochondria mirror the K m of NAD + -consuming enzymes localized to the corresponding compartment, hence these enzymes are sensitive to compartmental fluctuations in NAD + levels 77