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Future Foods, 4 , Article 100042

412 Ultimately, systemic inflammation and neuroinflammation promote each other, forming a persistent state of chronic low-grade inflammation, amplifying the damaging effect on neurons, and constituting a key driving mechanism for the pathological progression of diseases

Cycle threshold (Ct) values of target genes were calibrated to the Ct for the corresponding genes obtained for samples from the control pCYC1-GAP1 strain and normalised to the geometric mean of the reference genes for relative quantification using the comparative Ct method [36]

In addition, we found almost no change of OLFM4 level in MVs when the secretion of exosomes was blocked by N-SMase inhibitor GW4869, confirming MV-specific secretion of OLFM4 (Supplementary Fig