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These results confirmed that by upregulating the level of Bax/Bcl-2, the H 2 O 2 could promote the mitochondria to release cyt-C to the cytoplasm and activate caspase-9 and caspase-3 which are the downstream apoptotic proteins of mitochondrial pathway

Samples were mixed for 5 min and centrifuged again at 2000 rpm for an additional 5 min

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