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After the addition of three sample volumes of ice-cold methanol, mixing and centrifugation (20,000 g , 10 min, 4 C), the supernatant was removed and the pellet washed with three sample volumes of ice-cold methanol, mixed and centrifuged (20,000 g , 10 min, 4 C), followed by air-drying for around 10 min

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Freeman, J., Veggiotti, P., Lanzi, G., Tagliabue, A

Bloodbrain barrier disruption by lipopolysaccharide and sepsis-associated encephalopathy

Boehlke worked for many years as an ophthalmologist, dedicated to helping others including those in developing lands