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Transfection and cell surface staining of repRNAs in 293 cells repRNAs were transfected into 30 ml of 293 F cells at a density of 10 6 cells/ml in Freestyle 293 media (Thermo Fisher Cat

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RT-PCR Cell Proliferation/Viability Assay Cell Proliferation/Viability Assay Cell Proliferation/Viability Assay Cell Proliferation/Viability Assay All Endogenous Metabolite Isoforms More Biological Activity 50 & Target In Vitro L-Glutathione reduced is a non-protein thiol widely exists in living cells

NMR experiments NMR experiments were conducted on a Bruker Avance III spectrometer (Bruker, Ettlingen, Germany) operating at 600.13 MHz for 1 H observation