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The resulting reaction mixture was centrifuged at 15,000 g for 10 min, and the supernatant was analyzed by LC-FL (Waters, Milford, MA, USA) and LC-MS/MS (Shimazu, Kyoto, Japan)

Missiakas et al., 1996

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FACS analysis of yeast showed a gradual increase in biotinylation efficiency over the course of evolution, with LaccID being fourfold more active than the original template LacAnc100 (Fig