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A total of 31 targeted analytes were used for the multiplex kit, including inflammatory cytokines and chemokines: catalog number HCYTMAG-60K-PX30 (EGF, eotaxin, G-CSF, GM-CSF, IFN2, IFN, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-1RA, 1L-1, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, CXCL10, MCP-1, MIP-1, MIP-1, RANTES, TNF, TNF, and VEGF), and catalog number HADK2MAG-61K (NGF)

The gut microbiota-derived Pathogen-Associated Molecular Patterns, including LPS and peptidoglycan, are recognized by Pattern Recognition Receptors (PRRs), in particular Toll-like receptors and NOD-like receptors, which are expressed on osteoclasts and osteoblasts (236)