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Treatment of AMs with synthetic miR-21-5p mimics or miR-NC revealed that miR-21-5p significantly reduced the intracellular bacterial counts of Actinobacillus pleuropneumoniae (APP) when compared to miR-NC treatment( Figure 4D ) through a novel mechanism: the PIK3CD gene in AMs is targeted to activate the PI3K/Akt pathway, which in turn induces autophagy, this inhibits the overproduction of ROS and activates the NLRP3 inflammasome, preventing focal death and improving bacterial clearance

The inert matrix is typically composed of cross-linked agarose or polyacrylamide