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Using co-immunoprecipitation (CO-IP), the study confirmed that FOXO4-DRI prevents FOXO4 binding to p53, facilitating phosphorylated p53 nuclear exclusion, which triggers BAX upregulation and cleaved caspase-3 activation driving selective apoptosis of senescent endothelial cells

Researchers intending to work with the lyophilised version should ensure access to DMSO or a similar solvent for proper handling

Methionine is an essential amino acid that supports fat breakdown in the liver

More serious, though rare, side effects can occur, such as symptoms of low potassium levels (muscle cramps, weakness, irregular heartbeat) or signs of an allergic reaction

I53-50B.4PT1 expression and purification Lemo21 (DE3) (NEB) cells expressing I53-50B.4PT1 were grown in a 10 L BioFlo 320 Fermenter (Eppendorf) or a 2 L shake flask