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Busserolles J, Mazur A, Gueux E, Rock E, Rayssiguier Y

Abcam, 1:200 dilution) and fluorescent secondary antibodies (Alexa Fluor series, Invitrogen) for visualization

This optimal standard ensures a high concentration of active Glutathione, backed by rigorous clinical research for both efficacy and safety at this purity threshold

Other (less serious) concerns are if EGF can properly penetrate the skin (as it is a medium-sized, polar molecule, so a special delivery system is probably needed), if it can affect collagen synthesis (or just works on the surface plumping up only the upmost layers of the skin) and if it has beneficial effects at all when used in isolation versus when used in a "conditioned media" that contains lots of growth factors resembling the synergistic balance found in the skin

If inflammation is not controlled, it can affect deeper layers of the skin