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The theoretical maximum amount in solution is determined by the relative amounts of its pH-dependent unprotonated and protonated forms and follows the Henderson-Hasselbalch and precipitation-pH (pHp) models ( Figures 3 , and S4, S5, in Supplemental Information )

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[DOI] [PubMed] [Google Scholar] 49.Kitamura S., Sugihara K., Ohta S

In the above technical solution, in step 5, the freeze-drying comprises the following steps, preparing liquid and filling at 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 3h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 3h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the product is increased to 30 , preserving heat for 5 h