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Description

In severe cases it is possible to demonstrate free hemoglobin in plasma ( hemoglobinemia )

Jin F, Yang R, Wei Y, Wang D, Zhu Y, Wang X, et al

after reduction and alkylation using DTT and IAA, the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius), washed twice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and twice with 50 mM ammoniumbicarbonate