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doi: 10.1038/s41419-021-04315-1

For BrdU staining, the primary BrdU antibody was added together with 0.5 U l 1 DNAse (Sigma, D5025) and 1 mM MgCl 2 (Sigma, M8266) in the blocking solution and incubated for 30 min at room temperature

Same protocol with one less reconstitution

The above results suggested that GPX4 could be a target that explained, at least in part, the senolytic activity of SCLA1, SCLA2 and SCLA3

This means that cells lose pieces of their instruction manual and are unable to complete their function, which ultimately causes symptoms of ataxia-telangiectasia