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The samples were first loaded onto a Trap C18 column (100 m 20 mm, 5 m) and then separated on a C18 analytical column (75 m 150 mm, 3 m) using a gradient elution method at a flow rate of 300 nL/min

By employing lipid compositions having the desired fundamental properties, little or no energy need be added when mixing the lipid and an aqueous solution to form liposomes

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Antioxidants such as total phenolic content and tannin were observed to be significantly higher in cvs