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For SidC-mediated ubiquitination reaction 49 , 0.3 g GST-E1, 1 g His 6 -UbcH7, 3 g GST-SidC 1-542 and 4 g Ub were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 , 1 mM DTT and 2 mM ATP for 1 h at 37 C

For the estradiol treatment, 0.3 cm silastic estradiol capsules (Baxter HealthCare, Mundeleine, IL, USA) were implanted subcutaneously in the mice

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Magnetic Rack (for manual operation) Based on sample volume, the user can choose one of the following Magnetic Racks: BcMag Magnetic Rack-2 for holding two individual 1.5 ml centrifuge tubes (Cat

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