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15LO1 increases intracellular oxidative potential by lowering GSH and increasing extracellular GSSG To confirm that 15LO1-PEBP1 complex activity alters IL-13induced intracellular GSH levels, we modeled these relationships in vitro by stimulating cultured HAECs (at ALI) with IL-13 for 710 days in the presence and absence of 15LO1 protein (dicer siRNA knockdown [KD] of ALOX15 gene) or enzymatic activity (specific 15LO1 inhibitor BLX2477) (14, 15)

Emerging evidence for the widespread role of glutamatergic dysfunction in neuropsychiatric diseases

The cells were then collected for analysis in a BD FACSCanto II flow cytometer (BD Biosciences)

Gender and energy balance: sex differences in adaptations for feast and famine

10.3389/fgene.2021.673286 47 DubeyN.ChitranshiS.DwivediS