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The beads were washed 4 times with ice-cold RIPA buffer (50 mM HEPES, pH = 7.5, 500 mM LiCl, 1 mM EDTA, 1.0% NP-40, 0.7% Na-Deoxycholate), followed by once with ice-cold TE buffer (with 50 mM NaCl)

These data support a normal role for insulin signaling, as a determinant of chondrocyte size, which is apparently cannot compensated by local upregulation of IGF-1/IGF-1R

The apparatus comprised three chambers, two outer chambers of equal size, and a narrower centre chamber with a grid floor

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My local hospital said it was inoperable due to the size of the mass