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2 Dilute the resultant culture into 7H9 media containing 0.2% succinate and kanamycin at an OD 600 of 0.02, and incubate with shaking at 37 C to an OD 600 of 0.40.5 (~15 hr)

CSCs exhibit a resistance to radiation and chemotherapy due to the upregulation of numerous detoxifying enzymes, such as superoxide dismutase 2, glutathione peroxidases and heme oxygenase 1, that increase drug efflux and DNA repair capabilities (44,67,68)

Cyanocobalamin is the most widely available synthetic form, is shelf-stable, and is FDA-approved for treating B12 deficiency

Additional tests showed unconjugated hyperbilirubinemia (conjugated bilirubin: 34 mol/L

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