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In the TV series, the screenwriter, Lord Julian Fellowes, gives Dicky Merton a diagnosis of Pernicious Anaemia but in the next episode that is changed to iron deficiency anaemia

Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min

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Meet with the research staff member They will ask you structured questions about your health and any lupus symptoms youve experienced over the past 30 days

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