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However, a recent study revealed that iPSC-derived astrocytes obtained from C9orf72 ALS patients display increased oxidative stress and senescence (Birger et al., 2017)

Blots were incubated overnight at 4 C with primary antibodies and 2 h with HRP-conjugated secondary antibodies at room temperature

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It is understood that this is a result of long-range allosteric interactions between the methionine cycle and the folate cycle [18]