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Yang Z, Liu Y, Wang L, et al

400 l of the reagent mixture (30 ml glacial acetic acid, 20 ml phosphoric acid and 1.25 g ninhydrin) was mixed with 200 l supernatant and heated in sealed test tubes at 100 C for 1 h

This leads to an upper cervical (neck) subluxation that puts pressure on the brain stem

To investigate the ability of NDPIH to directly promote neurite outgrowth, we reduced further the number of neurons seeded to 10,000 per coverslip and omitted any serum during their culture