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The protein concentration was measured by BCA kit (Thermo Scientific TM Pierce TM BCA Protein Assay Kit, Waltham, MA, United States)

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Large population studies show that adults with lower carotenoid consumption tend to report shorter sleep duration, suggesting that antioxidant status may influence healthy sleep patterns.34 A smaller randomized trial tested 12mg of astaxanthin daily for eight weeks and found improvements in several sleep parameters, along with reduced perceived stress compared to placebo.4 The authors proposed that astaxanthins antioxidant and neuroprotective activity including its ability to cross the bloodbrain barrier could help stabilize oxidative stress in brain regions involved in regulating sleep and mood

Alterations in GSH metabolism and function have been implicated in various neurological aging while restoring redox balance and mitigating oxidative damage