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coli strains used originated or were derived from the KEIO collection (Baba et al., 2006), and were routinely cultivated at 37 C in Luria-Bertani (LB) medium, supplemented with antibiotics when required for plasmid maintenance and marker selection (ampicillin 200 g/mL or kanamycin 100 g/mL), if not stated differently

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Mol Cell (2019) 73:35463.e3

Funding This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery grant nos

Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids