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Parenteral Gln supplementation combined with enteral nutrition increased plasma and tissue Gln concentrations by upregulating the expression of heat shock protein 90 (Hsp90) and attenuated apoptosis in the lymphoid organ spleen and circulating lymphocytes, ultimately enhancing immune function and improving survival in severely burned rats (36)

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For western blotting, cells or tumour tissues were lysed in RIPA buffer