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These developments increase the challenges in the application of therapies to the most appropriate patient cohort, treatment combination, and timing

Additionally, the expression of tight junction-associated proteins in colonic tissues was significantly upregulated after the intervention, suggesting that the intervention may attenuate damage to intercellular tight junctions triggered by inflammatory factors, thereby protecting the intestinal mucosal barrier integrity

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Further, inhibition was dose-dependent, such that leukocyte adhesion was reduced with increases in the dose of oral 17-estradiol